7th Semester, Instrumental Method of Analysis, All units PDF

INSTRUMENTAL METHOD OF ANALYSIS








 UNIT -I

 Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications - Spectrophotometric titrations, Single component and multi component Analysis



Fluorimetry

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications. 


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UNIT –II 

IR spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations


Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell,
Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry-Principle, interferences, instrumentation and applications

Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications

Nepheloturbidometry- Principle, instrumentation and applications



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UNIT –III 

Introduction to chromatography

Adsorption and partition column chromatography-Methodology, advantages,

disadvantages and applications.

Thin layer chromatography- Introduction, Principle, Methodology, Rf values,

advantages, disadvantages and applications.

Paper chromatography-Introduction, methodology, development techniques,

advantages, disadvantages and applications

Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques

of paper, gel, capillary electrophoresis, applications

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UNIT –IV

Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications

High performance liquid chromatography (HPLC)-Introduction, theory, instrumentation, advantages and applications.

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UNIT –V 

Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications

Gel chromatography- Introduction, theory, instrumentation and applications

Affinity chromatography- Introduction, theory, instrumentation and applications

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